Figure 6

Up-regulation and nuclear localization of pax4 and arx altered transcript. (A–D) Confocal images of the pancreas area of 30 hpf embryos co-stained for pax4 mRNA (green) and Topro3 (blue) to visualize all cell nuclei, in control morphants (A-C) and pax4 morphants (B-D). Scale bar = 20 µm. (E-H) Confocal images of the pancreas area of 30 hpf embryos analyzed by fluorescent WISH with arx probe (red) and stained for Topro3 (blue). arx expression in somites and the floor plate of control morphants (E, G) and of arx morphants (F, H). Scale bar = 20 µm. (I-L) Ventral view of WISH 30 hpf embryos showing upregulation of arx transcripts in somites and floor plate in Moarx (J) and MoTarx (L) morphants. Original magnification 200x. (M – P) Ventral views of the pancreas area from embryos analyzed by WISH showing expression of pax4 in control morphants (M), pax4 morphants (N), arx morphants (O) and double pax4/arx morphants (P). Original magnification 400x. Anterior part of all the embryos is in the left. Asterisks indicate arx positive cells. fp: floor plate, s: somite, cont: control morphant.