Figure 5
Pax4 is not required for ß-cell differentiation. (A - J) Ventral views of the pancreas area from embryos analyzed by WISH, anterior to the left. Pancreatic expression of insulin (A, B), somatostatin (C, D), ghrelin (E, F), glucagon (G, H) and arx (I, J) in control morphants (A, C, E, F, I) and pax4 morphants (B, D, F, G, J) at 30 hpf. Quantifications (K, L, M, N) represent the number of positive cells per embryo for control morphants and pax4 morphants. Asterisks (*) indicate that the difference between cell number in control morphants and pax4 morphants is statistically significant by Student’s t-test (**: P<0.003; ***: P<0.0001). Original magnification 400x.