Table 1 Primer sequences used in molecular cloning and real-time PCR analysis
From: R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes)
Primer | Sequence | Purpose |
|---|---|---|
Rspo1-F1 | TGGGACTGGTGGCGCTGGCGATG | fragment amplification |
Rspo1-R1 | GCCTTTCTTAAACCCACATGT | |
Rspo2-F1 | ATGCAGTTTCGACTCTTCTC | |
Rspo2-R1 | TCACTGGCTGGAGCGAGCAG | |
Rspo3-F1 | GCCATGCAATTACAAGTCATCTC | |
Rspo3-R1 | CTACTGCACAAGGCTGTGCTCGGGG | |
Rspo1-F2 | CCCAGAAGGGAATAACCGATGCACAC | 3’- RACE |
Rspo1-F3 | CACACAGTGGGCGATGCTACGTCAGC | |
Rspo3-F2 | GGCAGACCCTGCCCTTTGACCACAG | |
Rspo3-F3 | GGCAGACCCTGCCCTTTGACCACAG | |
Rspo1-R2 | CGCTCCAAGAAGATAAAGAGCTTGGG | 5’- RACE |
Rspo1-R2 | CTCGTTTCTGCCTTCTCGCCTCCC | |
Rspo1-F4 | AAGTGCGTTGTGCCCAAAACACCG | real-time PCR |
Rspo1-R4 | TCCCATCTTTTCCCTCTCGCCCTAGTC | |
Rspo2-F2 | ACCACCCAAGGACACAATCC | |
Rspo2-R2 | GTGCTTCCCTGAACCACCTC | |
Rspo3-F4 | AAGAGGATCGGGATGAAGCA | |
Rspo3-R2 | TCACACTCCGACCTGCACTT | |
EF1-α-F | CAGCTTCAACGCTCAGGTCAT | |
EF1-α-R | TGAACTTGCAGGCGATGTGA | |
DMY-F | CCGGGTGCCCAA GTGCTCCCGCTG | genomic PCR for the genetic sex |
DMY-R | GATCGTCCCTCCACAGAGAAGAGA | Â |