Figure 7
Dependence on Skp1 expression level. Strains overexpressing Skp1A or Skp1B under control of either the ecmA or cotB promoter, in either a normal or phyA– background, were developed in submerged conditions beneath 100% O2 for 72 h. Typical results from selected strains are shown. (A) All tested strains formed tight aggregates except those overexpressing wild-type Skp1 in a phyA+ background, which remained as small, loose aggregates. (B) Spore numbers were counted and normalized to Ax3 spore counts in the same trial. Average values ± SEM from 2–3 independent trials are reported. Inset shows a Western blot for Skp1, showing its level of overexpression in the phyA– strains.