Figure 6
Dependence of spore differentiation on Skp1 glycosylation. (A) Schematic of the Skp1 modification pathway. Enzymes are in indicated by gene names; G= Gal; F= Fuc; Gn= GlcNAc. (B) Normal cells (Ax3) and modification pathway mutants were developed at 100% O2 in submerged conditions for 72 h. All strains formed similar tight aggregates, except that Ax3 aggregates exhibited dense cores; agtA– aggregates formed few spores in this trial. (C) Spore numbers were determined as in Figure 4. Average values ± SEM from 5 independent trials are shown. The wide error bar for agtA– cells results from a range of outcomes from near zero to more than Ax3.