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Figure 7 | BMC Developmental Biology

Figure 7

From: Ectopic expression of cyclase associated protein CAP restores the streaming and aggregation defects of adenylyl cyclase a deficient Dictyostelium discoideumcells

Figure 7

Ectopic expression of GFP-CAP induces DdCAD1 mRNA levels in aca- cells. Transcripts for the cell adhesion molecules DdCAD1 (A) and csA (B) were detected in AX2 and aca- cells and cells expressing GFP-CAP by northern blot analysis. 20 μg total RNA per time point (in hours, h) were used, probing was with DdCAD1 or csA cDNA probes. (C) Endogenous CAP and GFP-CAP mRNA levels were detected as controls. The increase in CAP mRNA amount in the aca- strain was not paralleled by an increase of the protein amounts (see Figure 1A and C, lanes 1, 2).

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BMC Developmental Biology

ISSN: 1471-213X

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