Figure 6

Quantitative agglutination assays of AX2 and aca^- cells expressing GFP-CAP or GFP-N-pro-CAP. 1 × 10⁷ cells/ml were starved in Soerensen phosphate buffer, collected at indicated times and incubated for an additional hour in the presence or absence of 10 mM EDTA. The OD600 was determined to measure the decrease in single cells as agglutination progresses in the presence or absence of EDTA. Agglutination is shown as percentages of the starting point (set to 100% which corresponds to essentially single cells in suspension). A decrease indicates aggregate formation. Agglutination of aca^- cells expressing GFP-CAP (A) or GFP-N-pro-CAP (B) in the absence and presence of EDTA was significantly improved. Data represents the mean of three independent experiments. Bars represent standard deviation. Agglutination of aca^- cells was compared to aca^- cells expressing GFP-CAP (A) or GFP-N-Pro-CAP (B) and statistics were performed using the Student's t-test and the P value < 0.05 is indicated with *.