Figure 5
Expression of GFP-CAP or GFP-N-pro-CAP in aca- cells promotes EDTA sensitive but not EDTA resistant cell adhesion. Phase contrast microscopy showing cell-cell adhesion of AX2 and aca- cells and AX2 and aca- null cells expressing GFP-CAP or GFP-N-pro-CAP in the presence or absence of 10 mM EDTA. 1 × 107 cells/ml were incubated with agitation (160 rpm/min) at 21°C. Samples were collected at indicated time points (in hours, h) and incubated in the presence or absence of EDTA for an additional hour. Images were obtained with a 20x bright field phase contrast microscope. White arrow points to small aggregates in aca- cells expressing GFP-CAP in the presence of EDTA. Bar, 100 μm.