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Figure 4 | BMC Developmental Biology

Figure 4

From: Ectopic expression of cyclase associated protein CAP restores the streaming and aggregation defects of adenylyl cyclase a deficient Dictyostelium discoideumcells

Figure 4

Ectopic expression of GFP-CAP influences csA protein levels in AX2 but not in aca- cells. Aggregation competent cells at a density of 1 × 107/ml were starved in Soerensen phosphate buffer. Samples were collected at the indicated times (hours, h) and lysed in 2 × SDS sample lysis buffer and resolved by 10% SDS-PAGE. Western blots were probed with csA specific mAb 33-294-17 (A) or with CAP specific antibody mAb 230-18-8 (B) as indicated. Expression of csA was enhanced in AX2 cells expressing GFP-CAP when compared to AX2. Arrow indicates the mature 80 kDa form of csA, the band below is a precursor. In aca- cells or transformants expressing GFP-CAP or GFP-N-pro-CAP no csA expression was detected at the protein level (C). Blots were reprobed with CAP antibody to reveal CAP and GFP-CAP fusions as indicated (D).

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BMC Developmental Biology

ISSN: 1471-213X

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