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Figure 9 | BMC Developmental Biology

Figure 9

From: Regulation of multiple tip formation by caffeine in cellular slime molds

Figure 9

The fate of cell types in secondary tips. A) The fate of ecmA (AX2-ecmA-GFP) during multiple tip formation at 3 h, 6 h and 12 h of development. Confined ecmA-GFP expression in the secondary tips. Scale bar = 200 μm. B) Fate of PspA (AX2-pspA-GFP) during multiple tip formation. The expression of PspA-GFP was monitored by taking pictures at 3 h, 6 h and 12 h of development. PspA-GFP expression in secondary tips and in fruiting bodies. Scale bar = 200 μm. The arrow indicates ecmA-GFP and pspA-GFP expression in the secondary tips. C) Semi-quantitative expression analysis (RT-PCR) of the ecmA and PspA mRNA in slugs having secondary tips. For comparative analysis of the transcript levels of ecmA and PspA, a semi-quantitative expression analysis was performed. Total RNA was extracted and cDNA was synthesized as described in material and methods. Equal amount of cDNA were used for all experiments and PCR was carried out for 35 cycles. Ig7 gene was used as an internal control. RT-PCR was performed from three independent samples. ecmA expression increased by three fold while PspA expression decreased by 50%. Primer sequences are mentioned in Table 2.

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