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Figure 5 | BMC Developmental Biology

Figure 5

From: Regulation of multiple tip formation by caffeine in cellular slime molds

Figure 5

Effect of IBMX (Iso-butyl methyl xanthine) on secondary tip formation. A) Effect of 0.8 mM IBMX on secondary tip formation in AX2 slugs. AX2 slugs formed secondary tips after 12 h of development in the presence of 0.8 mM IBMX. B) The neutral red stained slug of Pde4 mutants showed secondary tips at 9 h and 12 h of development. At 15 h, every culminant transformed into fruiting bodies. C) Morphology of the fruiting bodies: Ectopic tips were observed in pde4 fruiting bodies and in the wild type fruiting bodies developed in the presence of 2 mM caffeine and IBMX as indicated with arrows. Scale bar = 200 μm.

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