Figure 7
Endogenous GLI3 complexes bind to specific HMCO and GBR regions in the Grem1 genomic landscape. (A) Several amplicons were designed to detect the conserved and functionally relevant regions within the GRS1 region. The exact genomic locations of all amplicons are listed in Additional file 7. The potential interaction of endogenous GLI3 proteins with these critical amplicons was analysed by ChIP-qPCR analysis of wild-type (open bars) and Gli3Xt/Xt (black) limb buds at E11.5. Two stars indicate significant enrichment of amplicon “d” in the HMCO1 region (p = 0.008). (B) GLI3 ChIP-qPCR analysis of the HMCO2 region. Amplicons “f” and “g” are located inside HMCO2 and the previously identified GBR3 [32]. (C) GLI3 ChIP-qPCR analysis of HMCO3. All values are shown as mean ± SD.