Figure 9
Stimulation of cell invasion in OFT explants by knockdown of MBNL1 is TGFβ-mediated. (A) Stage 14 OFT explants were transfected with or without MBNL1 siRNA. An anti-TGFβ antibody or medium alone as added at 6 hrs and again after a boost at 18 hrs. The number of invaded cells was counted at 38 hrs. The mean number of invaded cells per explant + the standard error of the mean is shown. Inhibition of TGFβ repressed the enhancement of invasion in MBNL1 siRNA-treated explants to control levels. An asterisk indicates a significant difference from mock-transfected explants without antibody treatment (P ≤ 0.05). (B) Stage 14 OFT explants were treated with M199 supplemented with 0 or 200 ng/ml recombinant TGFβ3. The number of invaded cells was counted at 38 hrs. The mean number of invaded cells per explant + the standard error of the mean is shown. Addition of rTGFβ3 induced an increase in cell invasion in stage 14 OFT explants. An asterisk indicates a significant difference from 0 ng/ml rTGFβ3 (P ≤ 0.05).