Expression of canonical WNT/β-CATENIN signaling components in the developing human lung

Table 2 Primer sequences and amplicon sizes for PCR and in situ hybridization

Gene	Accession		Sequences (5’ → 3’)	Enzyme sites	Amplicon
WNT2	NM_003391	For	CCGCTCGAGTGGGAACAGTAAAGAAAGCAGAAT	XhoI	608 bp
		Rev	TGCTCTAGA TTAGCTCTGGAAACCTCTCTGTCA	XbaI
WNT7B	NM_058238	For	CCGCTCGAGCCCGGGGTGGCAGTAGGTAGC	XhoI	664 bp
		Rev	TGCTCTAGACAGCCATCCCCCTCTCCGGTAC	XbaI
FZD7	NM_003507	For	CCGCTCGAGCCTCCTGCGGTGTGCTTGTC	XhoI	519 bp
		Rev	TGCTCTAGACAACCAACGGGAAACCTCAGA	XbaI
LRP6	NM_002336	For	CCGCTCGAGGTGGAAGGGAATAATGGAAGC	XhoI	698 bp
		Rev	TGCTCTAGACCACCAGATCAAGATGCACATTTA	XbaI
DVL2	NM_004422	For	CCGCTCGAGGGGCGCTCCTGGTGTGTGAC	XhoI	434 bp
		Rev	TGCTCTAGAGCAGCTACATGGCCCAAATCTCC	XbaI
DVL3	NM_004423	For	CCGCTCGAGGGCACGCTCACTCCCTCATTCT	XhoI	464 bp
		Rev	TGCTCTAGATGCTCCAGGCCCAGGGTAAAT	XbaI
GSK-3β	NM_002093	For	CCGCTCGAGGGACTCCTGCCTCATGCCCCT	XhoI	527 bp
		Rev	TGCTCTAGAGCTCAGCCTGCTCAACACCCC	XbaI
APC	NM_001127511	For	CCGCTCGAGCGCGCTTACTGTGAAACCTGTT	XhoI	723 bp
		Rev	TGCTCTAGATTGCCTGTGGTCCTCATTTGTAG	XbaI
AXIN2	NM_004655	For	CCGCTCGAGGCCCGAAGCTCTTGTGAACTGTCT	XhoI	650 bp
		Rev	TGCTCTAGACGCAACATGGTCAACCCTCAAGA	XbaI
TCF4	NM_001083962	For	CCGCTCGAGGCTCGGCTGCCCTAGTAACAA	XhoI	678 bp
		Rev	TGCTCTAGATGCACACTACTTCGGCTACACAG	XbaI

ISSN: 1471-213X