Expression of canonical WNT/β-CATENIN signaling components in the developing human lung

Table 1 Primer sequences and amplicon sizes for real-time PCR

Gene	Accession		Sequences (5’ → 3’)	Length	Amplicon
WNT2	NM_003391	For	CTGACCTGATGCAGACGCAAG	21 bp	139 bp
		Rev	AGGAGCCACCTGTAGCTCTCATGTA	25 bp
WNT7B	NM_058238	For	GTCAGGGATGTTTGTCCCACTTG	23 bp	69 bp
		Rev	TCTGGTAGGTCCTTGTGCCACTC	23 bp
FZD4	NM_012193	For	TACCTCACAAAACCCCCATCC	21 bp	132 bp
		Rev	GGCTGTATAAGCCAGCATCAT	21 bp
FZD7	NM_003507	For	GCAAAGCAGCGCAAATCTGA	20 bp	116 bp
		Rev	AACCTCTGGCTTAACGGTGTGTG	23 bp
LRP5	NM_002335	For	ATGGGCGCCAGAACATCAA	19 bp	117 bp
		Rev	AGATGTCGATGCTGAGGTCGTG	22 bp
LRP6	NM_002336	For	TTGTTGCTTTATGCAAACAGACG	23 bp	167 bp
		Rev	CGTTTAATGGCTTCTTCGCTGAC	23 bp
DVL2	NM_004422	For	TGAGCAACGATGACGCTGTG	20 bp	148 bp
		Rev	GCAGGGTCAATTGGCTGGA	19 bp
DVL3	NM_004423	For	ACAATGCCAAGCTACCATGCTTC	23 bp	109 bp
		Rev	AGCTCCGATGGGTTATCAGCAC	22 bp
GSK-3β	NM_002093	For	TCGAGAGCTCCAGATCATGAGAA	23 bp	124 bp
		Rev	CGGAACATAGTCCAGCACCAGA	22 bp
CTNNB1	NM_001904	For	TCTGAGGACAAGCCACAAGATTACA	25 bp	122 bp
(β-CATENIN)		Rev	TGGGCACCAATATCAAGTCCAA	22 bp
APC	NM_001127511	For	CATGATGCTGAGCGGCAGA	19 bp	104 bp
		Rev	GCTGTTTCATGGTCCATTCGTG	22 bp
AXIN2	NM_004655	For	GAGTGGACTTGTGCCGACTTCA	22 bp	189 bp
		Rev	GGTGGCTGGTGCAAAGACATAG	22 bp
TCF4	NM_001083962	For	CTGCCTTAGGGACGGACAAAG	21 bp	101 bp
		Rev	TGCCAAAGAAGTTGGTCCATTTT	23 bp
LEF1	NM_016269	For	AATGAGAGCGAATGTCGTTGC	21 bp	137 bp
		Rev	GCTGTCTTTCTTTCCGTGCTA	21 bp
SOSTDC1	NM_015464	For	CTTGCCCCTGCCAGTGCTCC	20 bp	211 bp
		Rev	CTCGTTGTGCTGCCGGGTGT	20 bp
β-ACTIN	NM_001101	For	CATGTACGTTGCTATCCAGGC	21 bp	250 bp
		Rev	CTCCTTAATGTCACGCACGAT	21 bp

ISSN: 1471-213X