Figure 2

CalpB mutation delays border cell migration. Confocal micrographs of ( A) control ( w¹¹¹⁸) and (B) CalpB⁵⁰⁵/CalpB⁵⁰⁵ egg chambers at stage 10A are triple labeled with phalloidin-TRITC (red) for the actin network, with GFP (green) to show the slbo-Gal4 expression patterns and with DAPI (blue) for nuclei. The nurse cells (nc) and the oocyte (o) are indicated in panel A. The arrowheads show the positions of the border cell clusters, and the bars represent 30 μm The percentage scale represent the relative distances on the migration path between the anterior ends of the egg chambers and the oocytes. Note that in the wild-type egg chamber, the border cell cluster is located dorsally.