Figure 4

Potential crosstalk between histone citrullination and acetylation in early embryos. A. Colocalization of citrullinated H3R2 + 8 + 17 and acetylated H3K9. Two-cell embryos were immuno-stained with antibodies to H3Cit2 + 8 + 17 (red) and H3acK9 (green). The colocalization is highlighted in yellow in the merged panel. B. PN stage zygotes were co-cultured for ~68 hours in KSOM medium with 250 μM of Cl-amidine or 100 nM of TSA. Two or four-cell embryos were fixed and immuno-stained with antibodies to H3Cit 2 + 8 + 17 (red). DAPI was utilized to detect DNA (blue). DIC, differential interference contrast. Data are presented as mean + SEM. *P < 0.05, (two-tailed paired Student's t-test).