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Figure 1 | BMC Developmental Biology

Figure 1

From: Potential role for PADI-mediated histone citrullination in preimplantation development

Figure 1

Temporal and spatial distribution of histone H3 and H4 citrullination in oocytes and preimplantation embryos. A. Oocytes and embryos were isolated from CD1 female mice, fixed, permeabilized, and immunolabeled with antibodies against H4Cit3 (red). B. Same as (A) except anti-H3Cit2 + 8 + 17 antibodies (red) were used. Spindle apparatus and microtubule based mid-bodies are indicated by arrows and arrowheads, respectively. C. Same as (A) except anti-H3Cit26 antibodies (red) were used. Nucleoli are denoted by arrows. All samples were counterstained with 4′,6-diamidino-2-phyenylindole (DAPI) to visualize DNA (Blue). Cells were imaged by laser scanning confocal microscopy. DIC, differential interference contrast. PN, pronuclear. Blast., blastocyst.

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