Figure 1

“Tet On” regulated expression of the H2B-GFP fusion protein in the adult mouse esophagus. A) Schematic map of the ED-L2-rtTA fragment here developed and employed for transgenic mouse generation. B) Following doxycycline pulse, compound heterozygous ED-L2-rtTA/tetO-H2B-GFP transgenic animals express H2B-GFP. C-E) IHC analysis of H2B-GFP expression. After 7 days of doxycycline treatment (pulse), compound ED-L2-rtTA/tetO-H2B-GFP transgenic mice from all three founder lines (C. line 1; D. line 5, E. line 6, all 40x magnification) show strong nuclear H2B-GFP expression.