Table 2 Expression of wnts and frizzleds in OSE cells harvested from different animal ages and primer sequences for RT-PCR analysis
Gene | P5 | P10 | P21 | Adult (>6 wks old) | Forward primer | Reverse primer |
|---|---|---|---|---|---|---|
Wnt2 | + | + | + | + | CGACTGGGGTGGCTGCAGTG | AGGGAGCCTGCCTCTCGGTC |
Wnt2b | + | + | + | + | CATGATCAACGAGGGGACTT | CAGCCTTGTCCAAGACACAGT |
Wnt3a | – | – | – | – | CAAGCCGGCGATGGCTCCTC | ACTCCCGGGTGGCTTTGTCCA |
Wnt4 | + | + | + | + | GGCGTAGCCTTCTCACAGTC | GCCGTCAATGGCTTTAGATG |
Wnt5a | + | + | + | + | AGGAGTTCGTGGACGCTAGA | GTCCATCCCCTCTGAGGTCT |
Wnt7a | – | – | – | – | CTGGCGGGATCAGCACAGCC | CAGCCTCCCGACTCCCCACT |
Wnt8a | – | – | – | – | TCGTGGACAGTTTGGAGAAA | GTTGCGGTTGCAGTAGTCAG |
Wnt11 | + | + | + | + | CGTGTGCTATGGCATCAAGT | CGTGTGCTATGGCATCAAGT |
GAPDH | + | + | + | + | ACAACTTTGGCATTGTGGAA | GATGCAGGGATGATGTTCTG |
Fzd 1 | + | + | + | + | CCGAGCTCAAGTTCTTCCTG | GGGAACTTCTCGCACTTGAG |
Fzd 2 | + | + | + | + | TTCTTCACGGTCACCACCTA | AATGTAGGCCACTGACACCA |
Fzd 3 | + | + | + | + | CCAGGAACCTGACTTTGCTC | GACACTCCCTGCTTTGCTTC |
Fzd 4 | + | + | + | + | TGCAGTTCTTCCTTTGTTCG | TCTCAGGACTGGTTCACAGC |
Fzd 5 | + | + | + | + | GACGCCGAGGTTCTGTGTAT | TCGTTCCATGTCAATGAGGA |
Fzd 6 | + | + | + | + | CACAAATCATGGCACCTCTG | GGTTGGTTCTGGAGAACTGG |
Fzd 7 | + | + | + | + | GCCTACAACCAGACCATCCT | GCACACGGGTGCGTACATAG |
Fzd 8 | – | – | – | – | CCTTCGCCACTGTCTCTACC | ACGTGAGCGACAGGATTACC |
Fzd 9 | + | + | + | + | CCAGTACGTGGAGAAGAGTCG | GAAGGTGAACACGGTGAAGG |
Fzd 10 | + | + | + | + | GAGCACGGGCTGTACCTTAG | ATGAAGGAAGTGCCGATGAC |