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Figure 4 | BMC Developmental Biology

Figure 4

From: Expression of pair rule gene orthologs in the blastoderm of a myriapod: evidence for pair rule-like mechanisms?

Figure 4

Expression of h1 in the regio germinalis . All embryos are oriented with anterior to the left and represent ventral views, except panel (O), which represents a lateral view. (A) Early blastoderm stage embryo. Arrow points to enhanced expression at the posterior rim of the anterior expression domain. (B) Slightly older embryo as the one shown in (A). (C) Late blastoderm stage embryo. Arrow as in (A). Arrowhead points to enhanced expression at the anterior rim of the broad anterior expression domain. (D) Stage 0.1 embryo. A second broad expression domain covering the ocular region appears. The asterisk marks very faint expression in the maxillary segment. (E) Early stage 0.2 embryo. Asterisk as in (D). The arrow marks faint expression in the postmaxillary segment. (F) Late stage 0.2 embryo. The ocular domain of h1-expression has split. Asterisk as in (D). (G) Late stage 0.2 embryo double-stained for h1 (blue signal) and col (orange signal). Asterisk as in (D). (H) Stage 0.4 embryo. Asterisk as in (D). (H´) DAPI staining of the same embryo shown in (H). (I/J) Stage 0.4 embryos double-stained for h1 and en (both detected as blue signals). Note that a stripe of en-expression lies in the broad domain of h1-expression covering antennal to mandibular segments. Asterisks as in (D). (K) Stage 1.1 stained for h1. Arrowhead and arrow point to enhanced expression in the developing neuroectoderm. (L) Expression of h2 in a stage 0.1 embryo. Note that h2 is not expressed at the blastoderm stage. Arrow and arrowhead mark faint expression in the mandibular and maxillary segment respectively. (M) Stage 0.2 embryo stained for h2. Arrow points to upcoming expression in the postmaxillary segment. (N) Expression of h2 in a stage 0.4 embryo. (O) Co-expression of h2 (blue signal) and en (orange signal). h2 (blue arrowhead) is expressed anterior and adjacent to en (orange arrowhead). Abbreviations as in Figure 1.

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