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Figure 3 | BMC Developmental Biology

Figure 3

From: Expression of pair rule gene orthologs in the blastoderm of a myriapod: evidence for pair rule-like mechanisms?

Figure 3

Expression of run in the regio germinalis . All embryos are oriented with anterior to the left and represent ventral views, except panels (I) and (J) which represent lateral views. The run ortholog is expressed as two broad stripes in the early blastoderm stage (A, B), of which the posterior contributes to the first trunk segment (T1) (C). (D) Stage 0.1 embryo. The anterior broad stripe splits. (E) Early stage 0.2 embryo. (F) Late stage 0.2 embryo. (G) Early stage 0.3 embryo double-stained for run (blue signal) and col (orange signal; expression of col is marked with an orange arrowhead). (H) Late stage 0.3 embryo. Asterisk marks faint expression in the mandibular segment. Expression in the ocular segment (oc) appears. (I) Late stage 0.3 embryo double-stained for run (expression in the mandibular segment is marked with a blue arrowhead) and col (orange arrowhead). Asterisk as in (H). Lateral view. (J) Magnification of the embryo shown in (I). Arrowheads and asterisk as in (I). (K) Stage 0.4 embryo. Asterisk as in (I). (K´) DAPI staining of the embryo shown in (K). Abbreviations as in Figure 1.

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