Figure 4

Dose-dependent repression of Meis2, efnb1 and Pax7 by Pax3. (A-C) Meis2 expression in HH15 embryos electroporated with indicated concentrations of pMIWIII-Pax3. Loss of Meis2 transcripts can be seen in random patches of the electroporated right half of the mesencephalic vesicle only after transfection of 2 μg/μl of pMIWIII-Pax3 (C). (D) Loss of efnb1 transcripts following transfection of 2 μg/μl pMIWIII-Pax3. (E, F) Pax7 expression in the mesencephalic vesicle upon transfection of 1 μg/μl (E) and 2 μg/μl (F) pMIWIII-Pax3. The boxed areas in (B, E) are shown at a higher magnification in the upper right corners of the respective panels. (G) Quantification of the results: percent specimens with reduced (red bars) and unchanged (blue bars) expression of Meis2 (left) or Pax7 (right) following electroporation of different concentrations of pMIWIII-Pax3.