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Figure 2 | BMC Developmental Biology

Figure 2

From: Genetic and physical interaction of Meis2, Pax3 and Pax7 during dorsal midbrain development

Figure 2

Pax7 induces Pax3 expression in the mesencephalic vesicle. (A) Representative example of pMES-Pax7 electroporation into the mesencephalic vesicle. GFP expression, indicative of transgene expression, is restricted to the right half of the neural tube 4-6 hours later. (B-B") Flat mount preparation of a HH14 chick neural tube, 16 hours after electroporation of 2 μg/μl pMES-Pax7 into the mesencephalic vesicle; Pax3 expression is in dark blue, Nkx6.1 expression in pink, GFP in green. The dotted lines mark the dorso-ventral boundary, the arrow heads indicate representative examples of ectopic Pax7 positive cells co-expressing Pax3. Ectopic patches of Pax3 expression are restricted to the right, electroporated half of the mesencephalic vesicle. (B') is a higher magnification of (B) with the GFP fluorescent image superimposed onto the preparation. (B") shows the distribution of Pax7-GFP expressing cells in the specimen shown in (B'). (C) Quantification of the results: percent specimens with induced (green bars) and unaltered (blue bars) Pax3 expression following targeted electroporation of pMES-Pax7. (D) Flat mount preparation of a HH18 chick neural tube, 24 hours after electroporation of 2 μg/μl pMES-Pax7. (E) Targeted misexpression of pMES-Pax7 into the ventral mesencephalic vesicle showing cells ectopically expressing Pax3 within the Nkx6.1 domain. (E') is a higher magnification of (E). fb: forebrain; fp: floor plate; mes: mesencephalic vesicle; met: metencephalic vesicle; ov: optic vesicle; otv: otic vesicle. Scale bar (B, B'): 100 μm.

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