Figure 7
From: In vivo role of different domains and of phosphorylation in the transcription factor Nkx2-1
Expression of thyroglobulin, ZO-1, E-cadherin and Ksp-cadherin in foetal thyroid. Transversal sections of thyroid gland of wild type (A-D) and PM/PM (E-H) E18.5 embryos. Thyroid from wild type (A and B) and PM/PM (E and F) embryos were stained by double immunofluorescent with anti Tg (A, E) and anti ZO-1 (B, F) antibodies. In wild type embryos Tg is present in most, but not all, lumina (asterisks) and in the cells. Lumina are defined by the location of ZO-1 at the apical side of the cells. In PM/PM thyroids Tg staining is diffused in the gland and is not present in the empty spaces (E, circles) that do not have characteristics of lumina and have no localized ZO-1 staining (F). Wild type (C and D) and PM/PM (G and H) thyroids were also stained with anti E-cadherin and anti Ksp-cadherin antibodies. Membrane staining of E-cadherin was observed in both wild type and mutant thyroid (C and G). The Ksp-cadherin staining was much reduced in PM/PM thyroid (H). Asterisks indicate lumina. Bar 10 μm.