Figure 4From: Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryosConfirmation that the high-sedimenting materials contain polyribosomes. Cells were lysed and fractionated in the presence or absence of EDTA. RT-PCR was used to amplify the ACTB and CDK1 sequences from RNA isolated respectively (A) from granulosa cells and (B) from GV-stage oocytes. Top of gradient corresponds to fraction 1.Back to article page