BMP inhibition and FGF signaling prematurely induce the expression of early neural genes. (A-C) WISH for zicr1, foxd5α and sox2 of embryos injected with mRNA coding for Nog, FGF8a or Nog + FGF8a and lacZ mRNA (cyan) and collected at stage 8 (t = 6 hpf) and each subsequent hour after until stage 10.5 (t = 10 hpf) when cultured at room temperature. Red arrows indicate earliest onset of expression. All images are animal pole view with dorsal to the top. (D) RT-PCR of whole embryos dissected from uninjected embryos (UI) or embryos injected with Nog, FGF8a or Nog+FGF8a. Embryos were collected as in A-C. Genes analysed are indicated on left side, treatment on top, time of collection below panel. ODC used for loading control. All images are animal pole view with dorsal to the top.