Figure 3
From: Progenitor expansion in apc mutants is mediated by Jak/Stat signaling
Jak/Stat signaling mediates progenitor marker expression in the apc mutant hypothalamus. (A) Expression of ascl1b, a neural progenitor marker, is qualitatively increased in the apc mutant hypothalamus, and restored to wild-type levels by AG-490 treatment. Transverse cryosections at 36 hpf are shown. (B) Expression of Otx1/2, a hypothalamic progenitor marker, is increased in the apc mutant hypothalamus, and restored to wild-type levels by AG-490 treatment. Confocal projections from the ventral brain surface through the 36 hpf hypothalamus are shown. Otx1/2 immunohistochemistry is red, TO-PRO-3 nuclear stain is in blue. (C) The percent of Otx1/2+ cells is significantly increased in the apc mutant hypothalamus compared to wild-type siblings, and restored to wild-type levels by AG-490 treatment. Error bars = s.d., p < 0.05 by student's t-test.