Immunohistochemistry of DHH, PTCH1 and PTCH2 in the tammar wallaby ovary. DHH was found in the granulosa cells (GC) of follicles at all stages of development, and in the oocyte cytoplasm shown in primordial follicles (PrF). Staining was also observed for all three proteins in the cumulus cells (CC). PTCH1 and PTCH2 showed a similar distribution in the granulosa cells and were also present at reduced levels in the theca (T). PTCH2 staining was evident in the oocyte but PTCH1 was not. Scale bars indicate 160 μm, controls show immunohistochemistry with the primary antibody omitted.