Table 1 Accession number, primers and probes used for relative quantification of gene expression during gilthead sea bream ontogenic development and dietary vitamin A nutritional imbalance.
Gene name | Genebank | Component | 5' to 3' nucleotide sequences |
|---|---|---|---|
RARA | EU643830 | Forward | CCTGTCTGGACATCCTGATACTTC |
| Â | Â | Reverse | CGTGAGTCCATCTGAGAAAGTCAT |
| Â | Â | FAM probe | CTCTGGTGTGTAGCGTGTAC |
RARG | EU643831 | Forward | GTGCGTAATGACAGAAACAAGAAGA |
| Â | Â | Reverse | ACTCCTCTAGCTCTCCACTTAGC |
| Â | Â | FAM probe | CTTTCTGGAAGCACCACCTC |
RXRB | AM980430 | Forward | CCTGAGGCCCATGCAATCTC |
| Â | Â | Reverse | ACACACATGCGTTTCTGAGACAA |
| Â | Â | FAM probe | CAGCCCTGGACTAATG |
PPARA | AY590299 | Forward | CTTTTCGTGGCTGCCATTATCTG |
| Â | Â | Reverse | CTCCACCAAAGGCACATCCA |
| Â | Â | FAM probe | CCTGGGCGATCTCC |
PPARB | AY590301 | Forward | GTTTGTTGCTGCCATCATTCTCT |
| Â | Â | Reverse | CACCTGCTTCACGTTCATTAGC |
| Â | Â | FAM probe | CCGGGACGATCTCCAC |
PPARG | AY590304 | Forward | CAATGTCGGCATGTCACACAAC |
| Â | Â | Reverse | CTCCTTCTCCGCCTGGG |
| Â | Â | FAM probe | CCGGCCAAAACGAATG |
IGF1 | AY996779 | Forward | GGGCGAGCCCAGAGA |
| Â | Â | Reverse | GCCGTAGCCAGGTTTACTGAAATAA |
| Â | Â | FAM probe | TCCACACACAAACTGC |
IGF2 | AY996778 | Forward | GTCGGCCACCTCTCTACAG |
| Â | Â | Reverse | TGCTTCCTTGAGACTTCCTGTTTT |
| Â | Â | FAM probe | TTACCCGTGATGCCCC |
Bmp2 | AY500244 | Forward | GTGGCTTCCATCGTATCAACATTTT |
| Â | Â | Reverse | GCTCCCCGCCATGAGT |
| Â | Â | FAM probe | CAGGAGCTCCAAATAA |
TGFB1 | AF424703 | Forward | TTTTCCAACTTCGGCTGTACTGT |
| Â | Â | Reverse | GAGATGCCAAAACTGAAGGTACTGA |
| Â | Â | FAM probe | ATTGCGGCCGTTCTAG |
RBP | AY550957 | Forward | TGGCCACCTTCGAGACAAC |
| Â | Â | Reverse | GATGCGGCTCCCCAGTAG |
| Â | Â | FAM probe | CCCCGCCAAGTTCAG |
mgp | AY065652 | Forward | CGCCCGAAATACACCTCAGA |
| Â | Â | Reverse | GACGGACGGATACTAGGAGTCTA |
| Â | Â | FAM probe | ACCAGCCGCGACTCG |
bglap | AF048703 | Forward | CGAGCACATGATGGACACTGA |
| Â | Â | Reverse | GTCCGTAGTAGGCCGTGTAG |
| Â | Â | FAM probe | CAGCGATGATTCCC |
COL1A1 | DQ324363 | Forward | GGCAACAGTCGCTTCACCTA |
| Â | Â | Reverse | CCCCATGTACCGGTGTGT |
| Â | Â | FAM probe | ACGTGCATCCATCCTC |
SPP1 | AY651247 | Forward | CCAGCCAGGAGTCAGAGGAT |
| Â | Â | Reverse | ACTCTCATCTGAGTCGCTGTCA |
| Â | Â | FAM probe | CTGCTCTGGCTCTCC |
SPARC | AJ564190 | Forward | AAGCTGCACCTCGACTACATC |
| Â | Â | Reverse | CCTTCAGCTCGCTGTCCAT |
| Â | Â | FAM probe | CAGGGCTCGATCATTT |
EF1α | AF184170 | Forward | CCCGGTATGGTTGTCACCTT |
| Â | Â | Reverse | GGTGCATCTCCACAGACTTGAC |
| Â | Â | FAM probe | CCCCAGCTGACCACTG |
18S |  | TaqMan® Gene Expression Assay\Eukaryotic 18S rRNA, 20X (Part Number 4331182) | |