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Figure 8 | BMC Developmental Biology

Figure 8

From: Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A

Figure 8

Relative gene expression of RARA , RARB , RXRB and RBP (a), PPARA , PPARB and PPARG (b), Bmp2 , TGFB1 , IGF1 and IGF2 (c) and mgp , bglap , SPP1 , SPARC and COL1A1 (d) in fishes fed with experimental diets (1.5×VA and 10×VA, 1.00*108 and 6.82*108 total VA IU kg-1 DW, respectively) at 18 and 60 dph. Relative gene expression measured as the fold change of the target gene with respect to the house-keeping gene (EF1α) at the appropriate sample time and compared with gene expression in the control group using REST 2008 software. Gene up- and down-regulations are highlighted in red and green (respectively), using different colour tone to identify each experimental group and sampled time. Only significantly higher or lower overall gene expression levels are represented (P < 0.05; n = 3 per dietary group).

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