Gilthead sea bream bone mineralization at 60 dph of fishes from the different dietary treatments. Bone mineralization measured as ratios of red pixels (a), blue pixels per larval surface (b), and red pixels over blue pixels (c). Ratios are expressed as mean ± standard deviation. Letters denotes significant differences between dietary groups (ANOVA, P < 0.05; n = 26 larvae per treatment). C, larvae fed with control diet (0.66*108 total VA IU kg-1 DW); 1.5×VA, larvae fed with 1.5 fold increase in dietary VA content (1.00*108 total VA IU kg-1 DW); 10×VA, larvae fed with 10 fold increase in dietary VA content (6.82*108 total VA IU kg-1 DW).