Figure 8

Diagram of the apparatus used to superfuse 200-250 isolated Rana pipiens follicles loosely packed in a 10 mm diameter NMR tube containing modified Ringer's solution. Measurements were made at 20-22°C in Ringers solution with or without progesterone. Using two peristaltic pumps, follicles were continuously superfused by introducing aerated Ringer's solution at the bottom of the NMR tube (1 ml/min) and drawing medium off at the top of the tube. As shown, effluent was discarded and not recycled.