Figure 5

TBX3 over-expression is associated with increased mammary stem-like cell population. A and B) FACS analysis of mammary stem-like cells. (A) Representative graphs of gating strategies used to select Lin^- from Lin⁺ cell population. Anti-CD31, anti-CD45 and anti-TER119 antibodies were used to separate the Lin^- and Lin⁺ cells from harvested mammary glands of 12-week old female virgin mice (doxycycline induced and un-induced double transgenic littermates) (left panel). Gating strategy used to purify CD29^highCD24⁺ cells from Lin^- cell population (right panel). B) Statistical analysis of the average frequency of mammary stem-like cells (CD29^high, CD24⁺) in Lin^- population. Two mice from each group were used to generate the data. Error bars indicate SD; n = 2, *, P < 0.05.