Figure 2

MiR-184 promoted GC-1spg proliferation. A, Quantitative RT-PCR showed the increased levels of miR-184 upon overexpression. B, Flow cytometry analysis of GC-1spg transfected with miR-184-mimic (B-1) or scrambled oligonucleotide (B-2). C, MiR-184 overexpression resulted in significantly lower number of cells in the G1 phase (P < 0.001) and significantly higher number of cells in the S/G/M2 phase (P < 0.001) compared with the negative control. D, Analysis of cell proliferation using MTS assay. Y axis, measurement of A490, reflecting the relative cell number. X axis, units of time in days. Measurements were taken at days 0 to 3 after transfection of GC-1spg with miR-184-mimic (solid line) or scrambled negative control (dash line). This experiment was repeated three times in triplicate. Values are presented as the mean ± SD. **P < 0.001, *P < 0.01.