Figure 1

Expression pattern of miR-184. Upper panel, Relative quantity of miR-184 at different postnatal ages of mouse testes. X axis, different postnatal days of mouse testes; Y axis, miR-184 expression levels relative to postnatal day 7; Values are presented as the mean ± SD. Lower panel, Localization of miR-184 in adult mouse testes. MiR-184 (brown granules) was restricted to the cytoplasm of spermatogonia (S), spermatocytes (P) and round spermatids (R) in stage I-VI and stage VII-VIII, while the elongating or elongated spermatids (E) in stage IX-XI, stage I-VI were negative for miR-184. B-1 was a high magnification of the tubule in A-1 marked with (*). C-1 was the negative control using an LNA-modified, DIG-labeled scramble probe. Sections of A-1, B-1 and C-1 were counter-stained with hematoxylin as shown in A-2, B-2 and C-2. Roman numerals designate the stages of the seminiferous epithelium cycle: I-VI, stages I, II, III, IV, V or VI; VII-VIII, stages VII or VIII; IX-XI, stages IX, × or XI. Bars = 100 μm.