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Figure 1 | BMC Developmental Biology

Figure 1

From: Conserved and non-conserved enhancers direct tissue specific transcription in ancient germ layer specific developmental control genes

Figure 1

nkx3.2 CNEs and modified BAC. (A) UCSC browser snapshot showing the synteny around nkx3.2 and the functional CNE Bx4. (B) Whole mount RNA in situ hybridization showing expression of nkx3.2 in the sclerotome (SC) at 24 hpf. (C) Whole mount RNA in situ hybridization showing expression of nkx3.2 showing expression in branchial arch (BA) and sclerotome (SC) at 48 hpf. (D) Functional CNE Bx4 drives expression of EGFP in sclerotome (SC) at 24 hpf. (E) Modified BAC shows expression in both sclerotome and myotome (MY) at 24 hpf.

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