Figure 6

Double rbm24a and rbm24b morphants display more severe cardiac defects. Morpholinos directed to each rbm24 ortholog were injected individually and in tandem into zebrafish embryos and assessed at 24 hpf and 48 hpf compared to uninjected embryo hearts (A and E). Individual morpholino injections were titrated to a low dose for both rbm24a, 2.5 ng (B and F) and rbm24b, 4ng (C and G). Embryos receiving low doses of both rbm24a and rbm24b morpholinos resulted in more severe phenotype than either low dose MO alone, exhibiting severe cardiac defects and increased edema (D and H).