Nfatc3 protein levels are increased in both the nucleus and cytoplasm of Sox6-null myotubes. Wild type (WT) and Sox6-null (p100H) fetal primary myoblasts or myotubes were harvested at 0, 24, 48, 72, and 96 h after switching to DM, and nuclear and cytoplasmic protein was extracted for Nfatc3 Western blotting. TATA binding protein (TBP) and tubulin were used as loading controls for nuclear and cytoplasmic fractions, respectively. Upper panels: nuclear fractions. Lower panels: cytoplasmic fractions.