Table 1 Primers designed for analysis of TGIF1 &TGIF2 expression by RT-PCR
From: Differential roles of TGIF family genes in mammalian reproduction
Primers | Sequence (5'→3') | Function |
|---|---|---|
meTGIFF1 | CGAGACTGGCTCTATGAA | RT-PCR |
meTGIFR1 | AGATTTACCCGTGTCCTC | RT-PCR |
TF1 | TGAAGAT(C/T)CTCCGAGACTGG | Cross species cloning & RT-PCR |
TR1 | CCACCAGCAG(G/C/T)TGGAAGC | Cross species cloning & RT-PCR |
TF2 | ATGGCAAGGACCCTAACCG | 3' RACE and RT-PCR |
TR2 | TGGAAGAAGCCGCCGTCGTG | 5' RACE |
SMART IV | AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG | 5' RACE |
CDS III | ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N (N = A, G, C, or T; N-1 = A, G, or C) | 3' RACE |
5'PCR primer | AAGCAGTGGTATCAACGCAGAGT | 5' RACE |
18S F | GATCCATTGGAGGGCAAGTCT | RT-PCR |
18S R | CCAAGATCCAACTACGAGCTTTTT | RT-PCR |