Figure 5
From: Differential roles of TGIF family genes in mammalian reproduction
Semi-quantitative PCR. Identical amounts of cDNA template were utilized to identify the exponential (log) phase of the PCR reactions for TGIF1 and TGIF2 genes, while a 50 times cDNA dilution was used for amplification of the housekeeping gene 18S because of its high expression. Six tissues, adult testis, ovary and spleen as well as developing gonads and forebrain, were used for the analyses. PCRs were stopped at various cycles (15, 20, 25, 30, 35) to determine the exponential amplification phase.