Figure 1
scrib mutant cells expressing bskDN in the eye disc have impaired Hippo pathway signaling. Confocal sections through 3rd instar larval eye/antennal discs (A-F), posterior to the left in this and all subsequent figures. bskDN-expressing clones (A, C, E), and scrib1 clones expressing bskDN (B, D, F) were generated with ey-FLP and are positively marked by GFP expression (green, or magenta in the merges). Grayscale is DIAP1 (A-B), β-GAL (C-F), and F-actin to show tissue morphology (A-F). A white bar indicates the location of the morphogenetic furrow (MF) where cells are G1-phase arrested. (A, B) DIAP1 is expressed throughout the larval eye/antennal disc, and levels of the protein increase posterior to the MF in the differentiating portion of the eye disc. bskDN-expressing clones exhibit normal levels of DIAP1 (A), however, levels of DIAP1 are increased in scrib1 clones expressing bskDN (B). (C-F) ex-lacZ is expressed throughout the eye/antennal disc and is higher anterior to the MF in the eye disc; and fj-lacZ is expressed in a gradient in the eye disc with highest levels in the anterior centre, decreasing in levels along the dorsal, ventral and posterior axes. The expression of bskDN does not alter ex-lacZ (C) or fj-lacZ (E) expression, however, scrib1 clones expressing bskDN show elevated expression levels of ex-lacZ (D; note particularly clones of tissue posterior to the MF) and fj-lacZ (F).