Table 2 Applied antibodies and retrieval systems
From: Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver
Primary antibodies | Cat. # (clone) | Manufacturer | Dilution | Retrieval system |
|---|---|---|---|---|
CK7 | M7018 (OV-TL 12/30) | Dako | 1:50 | Retrieval solution, High pH* |
CK19 | M0888 (RCK108) | Dako | 1:50 | Retrieval solution, High pH* |
HepPar1 | M7158 (OCH1E5) | Dako | 1:35 | Retrieval solution, High pH* |
EpCAM | M0804 (BER-EP4) | Dako | 1:200 | Retrieval solution* |
Laminin | Z0097 | Dako | 1:500 | Proteinase K, ready to use+ |
SOX9 | HPA001758 | Sigma | 1:25 | Retrieval solution* |
AQP1 | AB3065 | Chemicon | 1:1200 | |
Nestin | MAB5326 (10C2) | Chemicon | 1:300 |