Transdifferentiation from cornea to lens in Xenopus laevis depends on BMP signalling and involves upregulation of Wnt signalling

Table 2 Q-rtPCR validation of 11 genes from the CRY list

Rank in array	Gene name	Lens (L/S)	CLT (R/S)
		qPCR	qPCR	array
1	Cryba1	37009.6	38.5	4.5
3	Crygb (sim)	75864.3	7.3	8.1
4	Crybb3	45918.7	7.8	6.2
6	Cryaa	95666.6	17.9	1.1
7	Glb1	7.6	0.8	1.3
8	Cugbp1b	41.8	1.3	2.4
15	Ccdc124	3.62	1.5	1.1
18	Crybb1	22333.3	5.0	9.6
26	Wnt7a	39193.7	0.5	1.1
29	Fzd7*	5.9	3.9	1.3/2.7
36	Kif19	2.1	0.5	1.2

Bold numbers indicate upregulation of more than 1.5 fold, numbers in italics indicate down regulation and normal font indicates no significant change. * appears twice in CRY list. L/S shows the relative (fold higher) expression in lens when compared to sham operated corneas. Likewise R/S shows relative expression in corneas undergoing CLT when compared to sham operated control corneas.

ISSN: 1471-213X