Figure 3

Co-detection of Arx and Pdx1, iAPP or PC1/3 in pancreata of wild type and Nkx2.2^-/- mice at E17.5. (A-C) Wild type Arx⁺ cells do not express Pdx1 (A) or PC1/3 (C, inset). Most Arx⁺ cells do not express iAPP (B), except in rare instances (Arrowhead). (D-F) The majority of Arx⁺ cells in Nkx2.2^-/- pancreata express Pdx1^low (Arrows), iAPP (E, inset) and PC1/3 (F, inset). Counterstaining with DAPI. Pictures were taken using confocal microscopy (Insets magnification: 4×).