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Table 1 Activation of genes by HNF1B in animal caps

From: The nephrogenic potential of the transcription factors osr1, osr2, hnf1b, lhx1 and pax8 assessed in Xenopus animal caps

 

gene symbol

fold induction

  

7 h (N = 5)

14 h (N = 4)

early nephrogenic TFs

lhx1

9.9 ↑

1.5

 

osr2

4.3 ↑

2.2

 

osr1

3.7 ↑

2.5

 

pax8

2.4

1.7

 

hnf1b

1.3

0.9

genes involved in nephrogenesis

hnf1a

13.7 ↑

7.1

 

wnt11b

4.6 ↑

1.3

 

wnt11

1.6

1.1

 

pax2

1.1

10.7 ↑

 

gdnf

1.2 ↑

1.4

 

wnt9b

0.8

0.7

proximal tubule genes

prodh2

95.9

1.7

 

hnf4a

22.9 ↑

3.4

 

anxa13

5.3

1.4

 

slc22a6

3.4

25.6

 

cpn1

2.3

3.1

 

tmem27

2.2

3.5

 

slc5a2

1.9

2.3

 

tfe3

1.8 ↑

1.2

 

rpl35a

1.5

1.5

 

gjb1

1.3

1.0

 

trps1

1.2

0.8

 

ube3a

1.1

0.8

 

esd

1.0

1.3 ↑

 

rbms1

1.0

0.2 ↓

 

slc7a8

1.0

1.5

 

fgfr4a

0.9

0.7

 

c8a

0.9

1.1

 

ncor1

0.9

1.1

 

slc4a7

0.9

1.1

 

fgfr4c

0.7

0.5 ↓

lhx1 target genes

cer1

421.3 ↑

2.9

 

chrd

9.0 ↑

7.8

 

pcdh8.2

5.1

2.0

 

gsc

3.5

0.1 ↓

 

otx2

1.3

1.3

  1. HNF1B (150 pg) injected animal caps were cultured for seven or fourteen hours in Steinberg's solution and analysed by quantitative RT-PCR for gene expression of the early nephrogenic transcription factors, of genes involved in nephrogenesis [78], of proximal tubule genes [40] and of the lhx1 target genes chrd [82], cer1 [38], gsc [82], otx2 [83] and pcdh8.2 [84] using the primers given in Additional file 2. Based on the low sample numbers (N = 4 or 5), genes were defined as induced or repressed, if all induction values were either higher or lower for a given probe. Distinct induction or reduction is indicated by an upward or downward arrow, respectively. N is the number of pools with 30 animal caps.