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Table 1 List of antibodies used in this study

From: Cytoskeleton proteins previously considered exclusive to Ganglion Cells are transiently expressed by all retinal neuronal precursors

Antibody

Antigen

Cell Type

Species

Indirect Fluorescent

Tyramide Amplification

Source

RA4

Microtubule Associated Protein (MAP)

Postmitotic Neuronal Precursors

Ganglion Cell axons

Mouse

ED3 1:500

ED3, ED6, ED7 1:10,000

ED8-ED18 1:2000

Kind gift from Dr. Steven McLoon

RMO270

Mid-sized Neurofilament (NF-M)

Postmitotic Neuronal Precursors

Adult Ganglion and Bullwhip Cells

Mouse

ED3-ED6 1:10,000

ED7-ED18 1:5,000

ED6 1:20,000

ED7-ED18 1:10,000

Kind gift from Dr. Virgnia Lee

3B5

AP2α

Amacrine and Horizontal Cells

Mouse

1:35

N/A

Developmental Studies Hybridoma Bank

Prox1

Prox1

Horizontal and Bipolar Cells

Rabbit

1:5,000

1:5,000

Millipore

4F2

Lim1/2

Horizontal Cells

Mouse

1:5

N/A

Developmental Studies Hybridoma Bank

67.4E12

Lim3

Bipolar Cells

Mouse

1:5

N/A

Developmental Studies Hybridoma Bank

Visinin

Visinin

Photoreceptor Cells

Rabbit

1:10,000

N/A

Kind gift from Dr. Leveillard

H5

Vimentin

Müller Glial Cells

Mouse

1:100

N/A

Developmental Studies Hybridoma Bank

39.3F7

Islet1

ED3-ED6: Ganglion Cells

ED7 and after: All Retinal Neuronal Cells

Mouse

1:50

1:5,000

Developmental Studies Hybridoma Bank

Hu C/D

Hu C/D

ED3-ED6: Ganglion Cells

ED7 and after: Ganglion and Amacrine Cells

Mouse

1:100

N/A

Invitrogen