Skip to main content

Advertisement

Table 1 List of antibodies used in this study

From: Cytoskeleton proteins previously considered exclusive to Ganglion Cells are transiently expressed by all retinal neuronal precursors

Antibody Antigen Cell Type Species Indirect Fluorescent Tyramide Amplification Source
RA4 Microtubule Associated Protein (MAP) Postmitotic Neuronal Precursors
Ganglion Cell axons
Mouse ED3 1:500 ED3, ED6, ED7 1:10,000
ED8-ED18 1:2000
Kind gift from Dr. Steven McLoon
RMO270 Mid-sized Neurofilament (NF-M) Postmitotic Neuronal Precursors
Adult Ganglion and Bullwhip Cells
Mouse ED3-ED6 1:10,000
ED7-ED18 1:5,000
ED6 1:20,000
ED7-ED18 1:10,000
Kind gift from Dr. Virgnia Lee
3B5 AP2α Amacrine and Horizontal Cells Mouse 1:35 N/A Developmental Studies Hybridoma Bank
Prox1 Prox1 Horizontal and Bipolar Cells Rabbit 1:5,000 1:5,000 Millipore
4F2 Lim1/2 Horizontal Cells Mouse 1:5 N/A Developmental Studies Hybridoma Bank
67.4E12 Lim3 Bipolar Cells Mouse 1:5 N/A Developmental Studies Hybridoma Bank
Visinin Visinin Photoreceptor Cells Rabbit 1:10,000 N/A Kind gift from Dr. Leveillard
H5 Vimentin Müller Glial Cells Mouse 1:100 N/A Developmental Studies Hybridoma Bank
39.3F7 Islet1 ED3-ED6: Ganglion Cells
ED7 and after: All Retinal Neuronal Cells
Mouse 1:50 1:5,000 Developmental Studies Hybridoma Bank
Hu C/D Hu C/D ED3-ED6: Ganglion Cells
ED7 and after: Ganglion and Amacrine Cells
Mouse 1:100 N/A Invitrogen