Bone morphogenetic protein-2 functions as a negative regulator in the differentiation of myoblasts, but not as an inducer for the formations of cartilage and bone in mouse embryonic tongue

Table 1 mRNA levels for genes related to myogenesis, chondrogenesis, and osteogenesis in vehicle- and BMP-2-treated tongues

Genes	Vehicle	Recombinant BMP-2	Significance
Myogenesis
MyoD	0.0163 ± 0.00616	0.0172 ± 0.00467	NS
Myogenin	0.0256 ± 0.00510	0.0180 ± 0.00162	p < 0.01
MCK	0.0661 ± 0.0149	0.0414 ± 0.00737	p < 0.01
Chondrogenesis, osteogenesis
Runx2	0	0.0000661 ± 0.0000800	NS
Osteocalcin	0.0000514 ± 0.0000552	0.0000581 ± 0.0000535	NS
ALP	0.000813 ± 0.000632	0.000700 ± 0.000576	NS
Collagen II	0.000167 ± 0.00246	0.000535 ± 0.0105	NS
Collagen X	0.000129 ± 0.00123	0.000155 ± 0.00168	NS

The mean ± standard deviation of six vehicle-treated or six BMP-2-treated tongues for each gene is shown. The ratio of each target gene mRNA relative to GAPDH mRNA is shown.

ISSN: 1471-213X