BMP-2 siRNA induced the expressions of myogenin and fMyHC in cultured tongue. Immunostaining images for myogenin (A, B) and fMyHC (C, D) in the middle portion of E13 tongues cultured for 8 days in BGJb medium containing 250 nM of the NTC (A, C) and BMP-2 siRNA (B, D). Brown color indicates immunostaining image for myogenin and fMyHC. Arrows in A and B indicate myogenin-positive nuclei. Western blotting pattern of myogenin, fMyHC, and βIII-tubulin in the whole portion of E13 tongues cultured for 8 days in BGJb medium containing the NTC and BMP-2 siRNA (E).