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Figure 5 | BMC Developmental Biology

Figure 5

From: Bone morphogenetic protein-2 functions as a negative regulator in the differentiation of myoblasts, but not as an inducer for the formations of cartilage and bone in mouse embryonic tongue

Figure 5

BMP-2 siRNA induced the expressions of myogenin and fMyHC in cultured tongue. Immunostaining images for myogenin (A, B) and fMyHC (C, D) in the middle portion of E13 tongues cultured for 8 days in BGJb medium containing 250 nM of the NTC (A, C) and BMP-2 siRNA (B, D). Brown color indicates immunostaining image for myogenin and fMyHC. Arrows in A and B indicate myogenin-positive nuclei. Western blotting pattern of myogenin, fMyHC, and ╬▓III-tubulin in the whole portion of E13 tongues cultured for 8 days in BGJb medium containing the NTC and BMP-2 siRNA (E).

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