Skip to main content
Figure 4 | BMC Developmental Biology

Figure 4

From: Bone morphogenetic protein-2 functions as a negative regulator in the differentiation of myoblasts, but not as an inducer for the formations of cartilage and bone in mouse embryonic tongue

Figure 4

BMP-2 siRNA suppressed the expression of BMP-2, but had neither inhibitory nor proliferative effects. Expression levels of BMP-2 (A) and BMP-4 (B) mRNAs in E13 tongues cultured for 8 days in BGJb medium containing 250 nM of NTC and BMP-2 siRNA. Immunostaining image for BMP-2 in the middle portion of E13 tongues cultured for 8 days in BGJb medium containing NTC (C) and BMP-2 siRNA (D). Western blotting pattern of BMP-2 and ╬▓III-tubulin in the whole portion of E13 tongues cultured for 8 days in BGJb medium containing the NTC and BMP-2 siRNA (E). Gross morphology of E13 tongues cultured for 8 days in BGJb medium containing NTC (F) and BMP-2 siRNA (G). Expression level of GAPDH mRNA in cultured E13 tongues (H). The longitudinal axis in H represents the quantity of GAPDH mRNA contained in one cultured tongue. Expression levels of cyclin D1 (I) and CDK4 (J) mRNAs in cultured E13 tongues. The longitudinal axis in I and J represents the ratio of target gene mRNA relative to GAPDH mRNA. Each column and vertical bar represent the mean + 1SD of six cultured tongues.

Back to article page